Dystrophin Antibody / DMD

ELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the Dystrophin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002% of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Amino acids 114-263 from the human protein were used as the immunogen for the Dystrophin antibody.

This Dystrophin antibody is available for research use only.

Cell surface, cytoplasmic

Protein G affinity chromatography

P11532