NSJ Bioreagents

Dystrophin Antibody / DMD

Product Code:
 
NSJ-V8443SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DMD/3677
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Dystrophin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC staining of FFPE human cardiac muscle with Dystrophin antibody (clone DMD/3677). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human skeletal muscle with Dystrophin antibody (clone DMD/3677). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Dystrophin antibody (clone DMD/3677) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human cardiac muscle with Dystrophin antibody (clone DMD/3677). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skeletal muscle with Dystrophin antibody (clone DMD/3677). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Dystrophin antibody (clone DMD/3677) as confirmation of integrity and purity.

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NSJ-V8443SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA: order Ab without BSA for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Dystrophin antibody should be determined by the researcher.
Description:
Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002% of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 114-263 from the human protein was used as the immunogen for the Dystrophin antibody.
Limitation:
This Dystrophin antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P11532

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