NSJ Bioreagents

E-Cadherin Antibody / CDH1

Product Code:
 
NSJ-V3702SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
ECD1-3R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R, green) and Reddot nuclear stain (red).~
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Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone ECD1-
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Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone ECD1-3R, green) and Reddot nuclear stain (red).~
Western blot testing of human MCF-7 cell lysate with recombinant E-Cadherin antibody (clone ECD1-

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NSJ-V3702SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.
Limitation:
This recombinant E-Cadherin antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity chromatography
Uniprot #:
P12830

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