NSJ Bioreagents

HER2 Antibody / ErbB2

Product Code:
 
NSJ-V7563SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ERBB2/3257
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the HER-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC staining of FFPE human breast cancer with HER-2 antibody (clone ERBB2/3257). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human breast cancer with HER-2 antibody (clone ERBB2/3257). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 4
SDS-PAGE analysis of purified, BSA-free HER-2 antibody (clone ERBB2/3257) as confirmation of integrity and purity.
4 / 4
Flow cytometry staining of PFA-fixed human MCF7 cells with HER-2 antibody; Red=isotype control, Blue= HER-2 antibody.
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IHC staining of FFPE human breast cancer with HER-2 antibody (clone ERBB2/3257). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with HER-2 antibody (clone ERBB2/3257). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free HER-2 antibody (clone ERBB2/3257) as confirmation of integrity and purity.
Flow cytometry staining of PFA-fixed human MCF7 cells with HER-2 antibody; Red=isotype control, Blue= HER-2 antibody.

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NSJ-V7563SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the HER-2 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human HER-2 protein fragment within amino acids 311-462 was used as the immunogen for the HER-2 antibody.
Limitation:
This HER-2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P04626

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