NSJ Bioreagents

Biotin / Vitamin B7 / Vitamin H Antibody

Product Code:
 
NSJ-V2319SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Hyb-8
Regulatory Status:
 
RUO
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of human tonsil with biotinylated lambda light chain antibody followed by anti-Biotin antibody (clone Hyb-8). Note cell membrane & cytoplasmic staining.~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free Biotin Antibody (clone Hyb-8).
❮❯

IHC staining of human tonsil with biotinylated lambda light chain antibody followed by anti-Biotin antibody (clone Hyb-8). Note cell membrane & cytoplasmic staining.~
SDS-PAGE Analysis of Purified, BSA-Free Biotin Antibody (clone Hyb-8).

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V2319SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Description:
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Limitation:
This Biotin antibody is available for research use only.
Localization:
Depends on target localization
Purity:
Protein G affinity chromatography

Documents