NSJ Bioreagents

c-Kit Antibody / CD117

Product Code:
 
NSJ-V2649SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KIT/983
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store the c-Kit antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot of a recombinant protein fragment with c-Kit antibody (KIT/983).~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free c-Kit Antibody (clone KIT/983). Confirmation of Integrity and Purity of the Antibody.
❮❯

Western blot of a recombinant protein fragment with c-Kit antibody (KIT/983).~
SDS-PAGE Analysis of Purified, BSA-Free c-Kit Antibody (clone KIT/983). Confirmation of Integrity and Purity of the Antibody.

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NSJ-V2649SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 2-4ug/ml
Application Note:
Optimal dilution of the c-Kit antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Description:
This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.
Limitation:
This c-Kit antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P10721

Documents