NSJ Bioreagents

Multi Cytokeratin Antibody

Product Code:
 
NSJ-V3699SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
KRT/1877R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of methanol-fixed human MCF7 cells with recombinant Multi Cytokeratin antibody antibody (clone KRT/1877R, green) and Reddot nuclear stain (red).~
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IHC staining of FFPE human skin with recombinant Multi Cytokerat
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Immunofluorescent staining of methanol-fixed human MCF7 cells with recombinant Multi Cytokeratin antibody antibody (clone KRT/1877R, green) and Reddot nuclear stain (red).~
IHC staining of FFPE human skin with recombinant Multi Cytokerat

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NSJ-V3699SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.
Limitation:
This recombinant Multi Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography

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