NSJ Bioreagents

KRT7 Antibody / Cytokeratin 7

Product Code:
 
NSJ-V7913SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KRT7/2200
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of FFPE human lung carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human breast carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human ovarian carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human placenta with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human HeLa cell lysate with Cytokeratin 8 antibody (clone KRT7/2200). Predicted molecular weight ~51 kDa.
6 / 6
SDS-PAGE analysis of purified, BSA-free Cytokeratin 17 antibody (clone KRT7/2200) as confirmation of integrity and purity.
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IHC staining of FFPE human lung carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HeLa cell lysate with Cytokeratin 8 antibody (clone KRT7/2200). Predicted molecular weight ~51 kDa.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 17 antibody (clone KRT7/2200) as confirmation of integrity and purity.

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NSJ-V7913SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. MAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant full-length human KRT7 protein was used as the immunogen for this Cytokeratin 7 antibody.
Limitation:
This Cytokeratin 7 antibody is available for research use only.
Purity:
Protein G affinity chromatography

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