NSJ Bioreagents

IgM Heavy Chain Antibody

Product Code:
 
NSJ-V7033SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MuHC2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot analysis of IgM antibody and Raji cell lysate (clone MuHC2). Expected molecular weight: 70-75 kDa.~
2 / 2
IHC analysis of FFPE human tonsil tissue and IgM antibody (clone MuHC2). Required HIER: boil sections in pH 9 10mM Tris with
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Western blot analysis of IgM antibody and Raji cell lysate (clone MuHC2). Expected molecular weight: 70-75 kDa.~
IHC analysis of FFPE human tonsil tissue and IgM antibody (clone MuHC2). Required HIER: boil sections in pH 9 10mM Tris with

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NSJ-V7033SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3507
Immunogen:
Human Mu heavy chain was used as the immunogen for this IgM antibody.
Limitation:
This IgM antibody is available for research use only.
Localization:
Cytoplasm, Cell Surface and Secreted
Purity:
Protein G

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