NSJ Bioreagents

Neurofilament Antibody Cocktail (Heavy + Light)

Product Code:
 
NSJ-V8380SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa + Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NF421 + NFL/736
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Neurofilament antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of permeabilized human HEK293 cells with Neurofilament antibody cocktail (green) and Palloidin (red).
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Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody cocktail (clone NF421 + NFL/736); Red=isotype control, Blue= Neurofilament antibody cocktail.
3 / 4
IHC staining of FFPE human cerebellum with Neurofilament antibody cocktail (NF421 + NFL/736). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE rat cerebellum with Neurofilament antibody cocktail (NF421 + NFL/736). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Immunofluorescent staining of permeabilized human HEK293 cells with Neurofilament antibody cocktail (green) and Palloidin (red).
Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody cocktail (clone NF421 + NFL/736); Red=isotype control, Blue= Neurofilament antibody cocktail.
IHC staining of FFPE human cerebellum with Neurofilament antibody cocktail (NF421 + NFL/736). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat cerebellum with Neurofilament antibody cocktail (NF421 + NFL/736). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

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NSJ-V8380SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Neurofilament antibody cocktail should be determined by the researcher.
Description:
This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human neurofilament protein was used as the immunogen for the Neurofilament antibody cocktail.
Limitation:
This Neurofilament antibody cocktail is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P12036

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