NSJ Bioreagents

CD57 / HNK-1 Antibody

Product Code:
 
NSJ-V2535SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgM kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NK/804
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD57 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD57 antibody (clone NK/804).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD57 antibody (clone NK/804).

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NSJ-V2535SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD57 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Membrane antigen from HSB-2 cells was used as the immunogen for the CD57 antibody.
Limitation:
This CD57 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
PEG precipitation
Uniprot #:
Q9P2W7

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