Product Code:
NSJ-V8204SAF
Antibody Isotype:
Mouse IgG2a kappa
Antibody Clonality:
Monoclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Functional Study
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
Storage:
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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This product comes from:
United States.
Typical lead time:
10-14 working days.
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Further Information
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (acetone fixed frozen sections),Immunofluorescence: 1-2ug/ml,Functional Studies (order BSA/azide-free format)
Optimal dilution of the antibody should be determined by the researcher.
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.
Purified
1 mg/ml in 1X PBS; BSA free, sodium azide free
Human PBMC were used as the immunogen for the CD3e antibody.
This CD3e antibody is available for research use only.
Cell surface, cytoplasmic
Protein G affinity chromatography
P07766