NSJ Bioreagents

PCNA Antibody

Product Code:
 
NSJ-V2775SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCNA/694
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694); Red=isotype control, Blue= PCNA antibody.~
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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PCNA/694).

Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694); Red=isotype control, Blue= PCNA antibody.~
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PCNA/694).

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NSJ-V2775SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the PCNA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full length human protein was used as the immunogen for the PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Localization:
Predominantly nuclear, some cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P12004

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