NSJ Bioreagents

Progesterone Receptor Antibody

Product Code:
 
NSJ-V2224IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PR484
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Progesterone Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human breast carcinoma stained with Progesterone Receptor antibody (clone PR484).~
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SDS-PAGE Analysis of Purified, BSA-Free Progesterone Receptor Antibody (clone PR484). Confirmation of Integrity and Purity of the Anti

IHC testing of FFPE human breast carcinoma stained with Progesterone Receptor antibody (clone PR484).~
SDS-PAGE Analysis of Purified, BSA-Free Progesterone Receptor Antibody (clone PR484). Confirmation of Integrity and Purity of the Anti

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NSJ-V2224IHC-7ML7 ml£534.00
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This product comes from: United States.
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Progesterone Receptor antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone. This progesterone receptor antibody is excellent for immunohistochemical staining of formalin/paraffin tissues.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
5241
Immunogen:
Recombinant human protein was used as the immunogen for this Progesterone Receptor antibody.
Limitation:
This Progesterone Receptor antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography

Documents