NSJ Bioreagents

Phosphotyrosine Antibody / P-Tyr

Product Code:
 
NSJ-V3095SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PY793
Regulatory Status:
 
RUO
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Phosphotyrosine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with Phosphotyrosine antibody (clone PY793).~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY793). Confirmation of Integrity and Purity of t

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with Phosphotyrosine antibody (clone PY793).~
SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY793). Confirmation of Integrity and Purity of t

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NSJ-V3095SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Phosphotyrosine antibody should be determined by the researcher.

1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues

Description:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This mAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
P-Tyr conjugated to BSA was used as the immunogen for the Phosphotyrosine antibody.
Limitation:
This Phosphotyrosine antibody is available for research use only.
Localization:
Depends upon the location of phosphorylated target
Purity:
Protein G affinity chromatography

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