NSJ Bioreagents

CD21 Antibody

Product Code:
 
NSJ-V7692SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rCR2/1952
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC staining of FFPE human spleen with recombinant CD21 antibody (clone rCR2/1952). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human spleen with recombinant CD21 antibody (clone rCR2/1952). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone rCR2/1952) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human spleen with recombinant CD21 antibody (clone rCR2/1952). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human spleen with recombinant CD21 antibody (clone rCR2/1952). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone rCR2/1952) as confirmation of integrity and purity.

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NSJ-V7692SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A partial protein corresponding to amino acids 44-196 was used as the immunogen for the recombinant CD21 antibody.
Limitation:
This recombinant CD21 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P20023

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