NSJ Bioreagents

GAPDH Antibody

Product Code:
 
NSJ-R20198BTN
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM114
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the recombinant GAPDH antibody at -20oC.
1 / 2
ICC staining of human HeLa cells using recombinant GAPDH antibody at 1:200 (red) and DAPI nuclear stain (blue).~
2 / 2
Western blot of human A431 cell lysate using the recombinant GAPDH antibody at 1:1000. Predicted molecular weight ~36
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ICC staining of human HeLa cells using recombinant GAPDH antibody at 1:200 (red) and DAPI nuclear stain (blue).~
Western blot of human A431 cell lysate using the recombinant GAPDH antibody at 1:1000. Predicted molecular weight ~36

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NSJ-R20198BTN-50UL50ul£524.00
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Further Information

Application Details:
Western Blot: 1:1000,Immunoprecipitation: 1:200,Chromatin IP (ChIP): 1:200,Immunocytochemistry: 1:200,Immunohistochemistry: 1:200 (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.
Format:
Biotin Conjugate
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of GAPDH was used as the immunogen for this recombinant GAPDH antibody.
Limitation:
This recombinant GAPDH antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P04406

Documents