NSJ Bioreagents

IgG Heavy Chain Antibody

Product Code:
 
NSJ-R20177
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM116
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
 
Store the recombinant Human IgG antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 5
Sandwich ELISA of human plasma using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains ( kappa;+ lambda;) antibody (<a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r30202btn>clone RM129</a>) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
2 / 5
Sandwich ELISA of human IgG using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains ( kappa;+ lambda;) antibody (<a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r30202btn>clone RM129</a>) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
3 / 5
IHC testing of FFPE human tonsil with recombinant Human IgG antibody.
4 / 5
ELISA of human immunoglobulins shows the recombinant Human IgG antibody reacted to the G1, G2, G3, G4 heavy chain of hIgGs, and the Fc of hIgG. No cross reactivity with other heavy chains or mouse/rat/goat IgG.
5 / 5
ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of the recombinant Human IgG antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA of human plasma using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains ( kappa;+ lambda;) antibody (<a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r30202btn>clone RM129</a>) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human IgG using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains ( kappa;+ lambda;) antibody (<a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r30202btn>clone RM129</a>) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human tonsil with recombinant Human IgG antibody.
ELISA of human immunoglobulins shows the recombinant Human IgG antibody reacted to the G1, G2, G3, G4 heavy chain of hIgGs, and the Fc of hIgG. No cross reactivity with other heavy chains or mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of the recombinant Human IgG antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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CodeSizePrice
NSJ-R20177-100UG100ug£524.00
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Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.5-2ug/ml (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Human IgG antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including G1, G2, G3, and G4. No cross reactivity with other human heavy chains or mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgG was used as the immunogen for this recombinant Human IgG antibody.
Limitation:
This recombinant Human IgG antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01857, P01859, P01860, P01861

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