NSJ Bioreagents

IgA Heavy Chain Antibody

Product Code:
 
NSJ-R20182
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM128
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
 
Store the recombinant Human IgA antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 4
Sandwich ELISA of human plasma using the recombinant Human IgA antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
2 / 4
Sandwich ELISA of human IgA using the recombinant Human IgA antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
3 / 4
ELISA of human immunoglobulins shows the recombinant Human IgA antibody reacts to both IgA1 & IgA2. No cross reactivity with IgG, IgM, IgD, or IgE.
4 / 4
ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
❮❯

Sandwich ELISA of human plasma using the recombinant Human IgA antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human IgA using the recombinant Human IgA antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows the recombinant Human IgA antibody reacts to both IgA1 & IgA2. No cross reactivity with IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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CodeSizePrice
NSJ-R20182-100UG100ug£524.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgA antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgA was used as the immunogen for this recombinant Human IgA antibody.
Limitation:
This recombinant Human IgA antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01876, P01877

Documents