PDCD1 Antibody / PD-1 / PD1

ELISA (order BSA/azide-free format for coating),Neutralization Studies (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml

Optimal dilution of the antibody should be determined by the researcher.

PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1 mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells).

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

PDCD1 transfected BHK cells were used as the immunogen for the PD1 antibody.

This PD1 antibody is available for research use only.

Protein G affinity chromatography

Q02242