RPA32 Antibody / Replication Protein A2

Western blot: 1-2ug/ml

Optimal dilution of the RPA32 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Full length recombinant human protein was used as the immunogen for this RPA32 antibody.

This RPA32 antibody is available for research use only.

Nuclear

Protein G affinity chromatography

P15927