NSJ Bioreagents

PGP9.5 / UchL1 Antibody

Product Code:
 
NSJ-V9096SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM574
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the anti-PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Immunofluorescent staining of permeabilized human T98G cells with anti-PGP9.5 antibody cocktail (green, clone SPM574) and Nucspot (red).
2 / 3
IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with anti-PGP9.5 antibody (clone SPM574).
3 / 3
Western blot testing of human brain lysate with anti-PGP9.5 antibody (clone SPM574). Predicted molecular weight ~25 kDa.
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Immunofluorescent staining of permeabilized human T98G cells with anti-PGP9.5 antibody cocktail (green, clone SPM574) and Nucspot (red).
IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with anti-PGP9.5 antibody (clone SPM574).
Western blot testing of human brain lysate with anti-PGP9.5 antibody (clone SPM574). Predicted molecular weight ~25 kDa.

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NSJ-V9096SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Native UchL1/PGP9.5 protein isolated from human brain was used as the immunogen for this anti-PGP9.5 antibody.
Limitation:
This anti-PGP9.5 antibody is available for research use only.
Localization:
Cytoplasmic. Endoplasmic Reticulum membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P09936

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