MUC6 Antibody / Gastric Mucin

Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the anti-MUC6 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

The MUC6 gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A synthetic peptide of the gastric Mucin 6 tandem repeat sequence was used as the immunogen for the anti-MUC6 antibody.

This anti-MUC6 antibody is available for research use only.

Cytoplasmic & secreted

Protein G affinity chromatography

Q6W4X9