NSJ Bioreagents

CD43 Antibody

Product Code:
 
NSJ-V3651SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
SPN/2049R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC testing of FFPE human tonsil with recombinant CD43 antibody (clone SPN/2049R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CD43 antibody (clone SPN/2049R) as confirmation of integrity and purity.
3 / 3
FACS staining of PFA-fixed human K562 cells with recombinant CD43 antibody (clone SPN/2049R). Red=isotype control, Blue= recombinant CD43 antibody.
❮❯

IHC testing of FFPE human tonsil with recombinant CD43 antibody (clone SPN/2049R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CD43 antibody (clone SPN/2049R) as confirmation of integrity and purity.
FACS staining of PFA-fixed human K562 cells with recombinant CD43 antibody (clone SPN/2049R). Red=isotype control, Blue= recombinant CD43 antibody.

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NSJ-V3651SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant CD43 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant CD43 antibody.
Limitation:
This recombinant CD43 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
P16150

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