Tenascin C Antibody

Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the Tenascin C antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this mAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human breast carcinoma cells were used as the immunogen for the Tenascin C antibody.

This Tenascin C antibody is available for research use only.

Connective tissue matrix

Protein G affinity chromatography

P24821