NSJ Bioreagents

CD45RO Antibody (T-cell marker)

Product Code:
 
NSJ-V2237IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
UCHL-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD45RO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of human tonsil stained with CD45RO antibody (UCHL-1).~
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SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone UCHL-1). Confirmation of Integrity and Purity of the Antibody.

IHC testing of human tonsil stained with CD45RO antibody (UCHL-1).~
SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone UCHL-1). Confirmation of Integrity and Purity of the Antibody.

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NSJ-V2237IHC-7ML7 ml£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
5788
Immunogen:
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody.
Limitation:
This CD45RO antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography

Documents