NSJ Bioreagents

CD3 epsilon Antibody

Product Code:
 
NSJ-V3760SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
UCHT1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Storage:
 
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of human Jurkat cells with CD3e antibody (clone UCHT1, green) and Reddot nuclear stain (red).~
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Flow cytometry testing of human Jurkat cells with CD3e antibody (clone UCHT1); Red=isotype control, Blue= CD3e anti

Immunofluorescent staining of human Jurkat cells with CD3e antibody (clone UCHT1, green) and Reddot nuclear stain (red).~
Flow cytometry testing of human Jurkat cells with CD3e antibody (clone UCHT1); Red=isotype control, Blue= CD3e anti

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NSJ-V3760SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (Acetone-fixed frozen tissues): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD3e antibody should be determined by the researcher.
Description:
This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor were used as the immunogen for the CD3e antibody.
Limitation:
This CD3e antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P07766

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