VEGFA Antibody / Vascular Endothelial Growth Factor A

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the VEGF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.

This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human recombinant protein was used as the immunogen for the VEGF antibody.

This VEGF antibody is available for research use only.

Cytoplasmic, cell surface and extracellular (secreted)

Protein G affinity chromatography

P15692