NSJ Bioreagents

Vimentin Antibody

Product Code:
 
NSJ-V8356SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
VIM/1937R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 5
IHC staining of FFPE human tonsil with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human colon carcinoma with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human melanoma with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human A375 and U-87 cell lysate with Vimentin antibody (clone VIM/1937R). Expected molecular weight: 53-58 kDa.
5 / 5
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VIM/1937R) as confirmation of integrity and purity.

IHC staining of FFPE human tonsil with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma with recombinant Vimentin antibody (clone VIM/1937R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human A375 and U-87 cell lysate with Vimentin antibody (clone VIM/1937R). Expected molecular weight: 53-58 kDa.
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VIM/1937R) as confirmation of integrity and purity.

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NSJ-V8356SAF-100UG100 ug£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant protein was used as the immunogen for this recombinant Vimentin antibody.
Limitation:
This recombinant Vimentin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P08670

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