NSJ Bioreagents

VISTA Antibody / GI24

Product Code:
 
NSJ-V7580IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
VISTA/3007
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the VISTA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of FFPE human squamous cell carcinoma with VISTA antibody (clone VISTA/3007). HIER: boil tissue sections in pH 9, 10mM Tris with 1mM EDTA, 10-20 min and allow to cool before testing.~
2 / 2
IHC staining of FFPE human placenta with

IHC staining of FFPE human squamous cell carcinoma with VISTA antibody (clone VISTA/3007). HIER: boil tissue sections in pH 9, 10mM Tris with 1mM EDTA, 10-20 min and allow to cool before testing.~
IHC staining of FFPE human placenta with

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NSJ-V7580IHC-7ML7 ml£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the VISTA antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC's, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30kDa extracellular fragment plus a 25-30kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
A recombinant full-length human VISTA protein was used as the immunogen for the VISTA antibody.
Limitation:
This VISTA antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
Q9H7M9

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