NSJ Bioreagents

MART-1 / Melan-A Antibody

Product Code:
 
NSJ-V3278SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
2 / 2
Western blot testing of human COLO-38 cell lysate with Melan-A antibody. Expected molecular weight ~20
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IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
Western blot testing of human COLO-38 cell lysate with Melan-A antibody. Expected molecular weight ~20

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NSJ-V3278SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.
Description:
MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human MART-1/Melan-A protein was used as the immunogen for this MART-1 antibody.
Limitation:
This MART-1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
Q16655

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