NSJ Bioreagents

CD38 Antibody

Product Code:
 
NSJ-V9180SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CD38/6448R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant CD38 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC staining of FFPE human prostate adenocarcinoma tissue with recombinant CD38 antibody (clone CD38/6448R) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with recombinant CD38 antibody (clone CD38/6448R) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Negative control: IHC staining of FFPE human brain tissue with recombinant CD38 antibody (clone CD38/6448R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human Raji cell lysate using recombinant CD38 antibody (clone CD38/6448R). Expected molecular weight: 34-46 kDa depending on glycosylation level.
5 / 5
SDS-PAGE analysis of purified, BSA-free recombinant CD38 antibody (clone CD38/6448R) as confirmation of integrity and purity.
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IHC staining of FFPE human prostate adenocarcinoma tissue with recombinant CD38 antibody (clone CD38/6448R) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD38 antibody (clone CD38/6448R) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD38 antibody (clone CD38/6448R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate using recombinant CD38 antibody (clone CD38/6448R). Expected molecular weight: 34-46 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free recombinant CD38 antibody (clone CD38/6448R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9180SAF-100UG100ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant CD38 antibody should be determined by the researcher.
Description:
CD38 is a type II transmembrane glycoprotein that is present on early B- and T-cell lineages and activated B- and T-cells but is absent from most mature resting peripheral lymphocytes. CD38 is found on thymocytes, pre-B cells, germinal center B-cells, mitogen-activated T-cells, monocytes and Ig-secreting plasma cells. CD38 is expressed on CD34+ cells. The CD34+CD38- population of hematopoietic stems cells define the most pluripotent cells (e.g.blast colony forming cells).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 200-300 of human CD38 protein was used as the immunogen for the recombinant CD38 antibody.
Limitation:
This recombinant CD38 antibody is available for research use only.
Localization:
Cell Surface, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P28907

Documents