Elabscience

NR3C2 Polyclonal Antibody

Product Code:
 
E-AB-93380
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines using Mineralocorticoid receptor Polyclonal Antibody at 1:1000 dilution.
2 / 4
Immunohistochemistry of paraffin-embedded human liver cancer using Mineralocorticoid receptor Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 4
Immunofluorescence analysis of C6 cells using Mineralocorticoid receptor Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH-3T3 cells using Mineralocorticoid receptor Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using Mineralocorticoid receptor Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver cancer using Mineralocorticoid receptor Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Mineralocorticoid receptor Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Mineralocorticoid receptor Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-93380-60uL60uL£210.00
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E-AB-93380-120uL120uL£291.00
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E-AB-93380-200uL200uL£431.00
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Further Information

Abbreviation:
NR3C2
Background:
This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
75kDa/94kDa/107kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Mineralocorticoid receptor
ObservedMW:
100KDa
Purification method:
Affinity purification
Target Synonym:
NR3C2;MCR;MLR;MR;NR3C2VIT
UNIProt ID:
P08235

Documents