Elabscience

(KO Validated) NF-kB p65 Polyclonal Antibody

Product Code:
 
E-AB-93366
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Western blot analysis of various lysates using NF-kB p65/RelA Polyclonal Antibody at 1:2000 dilution.
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Western blot analysis of extracts from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa(KO) cells using NF-kB p65/RelA Polyclonal Antibody at 1:2000 dilution.
3 / 4
Immunofluorescence analysis of HepG2 cells using [KO Validated] NF-kB p65/RelA Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] NF-kB p65/RelA Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of various lysates using NF-kB p65/RelA Polyclonal Antibody at 1:2000 dilution.
Western blot analysis of extracts from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa(KO) cells using NF-kB p65/RelA Polyclonal Antibody at 1:2000 dilution.
Immunofluorescence analysis of HepG2 cells using [KO Validated] NF-kB p65/RelA Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] NF-kB p65/RelA Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-93366-60uL60uL£224.00
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E-AB-93366-120uL120uL£317.00
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E-AB-93366-200uL200uL£465.00
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Further Information

Abbreviation:
NF-kB p65
Background:
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
58kDa/59kDa/60kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human NF-kB p65/RelA
ObservedMW:
65KDa
Purification method:
Affinity purification
Target Synonym:
RELA; NFKB3; p65; transcription factor p65
UNIProt ID:
Q04206

Documents