Elabscience

(KO Validated) N-Cadherin Polyclonal Antibody

Product Code:
 
E-AB-93279
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Western blot analysis of extracts of various cell lines using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of HeLa cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of 293T cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts from wild type(WT) and N-Cadherin knockout (KO) HeLa cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
5 / 6
Immunohistochemistry of paraffin-embedded Human colon carcinoma using N-Cadherin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin Polyclonal Antibody. Western blot was performed from the immunoprecipita
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Western blot analysis of extracts of various cell lines using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of HeLa cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of 293T cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and N-Cadherin knockout (KO) HeLa cells using N-Cadherin Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human colon carcinoma using N-Cadherin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin Polyclonal Antibody. Western blot was performed from the immunoprecipita

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E-AB-93279-60uL60uL£224.00
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E-AB-93279-200uL200uL£465.00
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Further Information

Abbreviation:
N-Cadherin
Background:
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
97kDa/99kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IP 1:50-1:200
Immunogen:
A synthetic peptide of human N-Cadherin
ObservedMW:
140KDa
Purification method:
Affinity purification
Target Synonym:
CDH2; CD325; CDHN; CDw325; NCAD; cadherin-2
UNIProt ID:
P19022

Documents