Elabscience

Ubiquitin Polyclonal Antibody

Product Code:
 
E-AB-93277
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Western blot analysis of extracts of various cell lines using Ubiquitin Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human placenta using Ubiquitin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using Ubiquitin Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human placenta using Ubiquitin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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E-AB-93277-60uL60uL£210.00
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E-AB-93277-120uL120uL£291.00
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E-AB-93277-200uL200uL£431.00
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Further Information

Abbreviation:
Ubiquitin
Background:
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
25kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human Ubiquitin
ObservedMW:
17-110kDa
Purification method:
Affinity purification
Target Synonym:
HEL-S-50;Ubiquitin;UBB
UNIProt ID:
P0CG47

Documents