Elabscience

(KO Validated) SUMO1 Polyclonal Antibody

Product Code:
 
E-AB-92410
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Immunohistochemistry of paraffin-embedded human lung cancer using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
2 / 4
Immunohistochemistry of paraffin-embedded mouse testis using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 4
Immunofluorescence analysis of C6 cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U2OS cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human lung cancer using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using SUMO1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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E-AB-92410-60uL60uL£224.00
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E-AB-92410-120uL120uL£317.00
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E-AB-92410-200uL200uL£465.00
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Further Information

Abbreviation:
SUMO1
Background:
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
8kDa/11kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
A synthetic peptide of human SUMO1
ObservedMW:
16KDa/80KDa
Purification method:
Affinity purification
Target Synonym:
SUMO1; DAP1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; UBL1; small ubiquitin-like modifier 1
UNIProt ID:
P63165

Documents