Elabscience

PDE4A Polyclonal Antibody

Product Code:
 
E-AB-92180
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 3
Immunohistochemistry of paraffin-embedded rat kidney using PDE4A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
2 / 3
Immunofluorescence analysis of K-562 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat kidney using PDE4A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of K-562 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-92180-60uL60uL£210.00
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E-AB-92180-120uL120uL£291.00
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E-AB-92180-200uL200uL£431.00
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This product comes from: China.
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Further Information

Abbreviation:
PDE4A
Background:
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
98kD/95kDa/76kDa/72kDa/37kDa/91kDa/96kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:1000-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human PDE4A
ObservedMW:
80KDa/110KDa
Purification method:
Affinity purification
Target Synonym:
PDE4; DPDE2; PDE46
UNIProt ID:
P27815

Documents