Elabscience

LC3B Polyclonal Antibody

Product Code:
 
E-AB-68372
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various lysates using LC3B Polyclonal Antibody at 1:2000 dilution.NIH/3T3 and C6 were treated by Chloroquine (50 uM) at 37°C for 20 hours.
2 / 2
Immunohistochemistry of paraffin-embedded Human breast cancer using LC3B Polyclonal Antibody at dilution of 1:250 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various lysates using LC3B Polyclonal Antibody at 1:2000 dilution.NIH/3T3 and C6 were treated by Chloroquine (50 uM) at 37°C for 20 hours.
Immunohistochemistry of paraffin-embedded Human breast cancer using LC3B Polyclonal Antibody at dilution of 1:250 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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E-AB-68372-60uL60uL£210.00
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E-AB-68372-120uL120uL£291.00
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E-AB-68372-200uL200uL£431.00
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Further Information

Abbreviation:
LC3B
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
14kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human LC3B
ObservedMW:
14kDa/16kDa
Purification method:
Affinity purification
Target Synonym:
LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
UNIProt ID:
Q9GZQ8

Documents