Human Ang-Ⅰ(Angiotensin Ⅰ) ELISA Kit

Ang-I

2.5h

This kit recognizes Human Ang-I in samples.No significant cross-reactivity or interference between Human Ang-I and analogues was observed

125-8000 pg/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

75 pg/mL

Human

Angiotensin-1

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-I. During the reaction, Human Ang-I in the sample or standard competes with a fixed amount of Human Ang-I on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-I. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Human Ang-I in tested samples can be calculated by comparing the OD of the samples to the standard curve.

serum, plasma and other biological fluids

Competitive

P01019

This ELISA kit applies to the in vitro quantitative determination of Human Ang-I concentrations in serum, plasma and other biological fluids.