Elabscience

Caspase-9 Polyclonal Antibody

Product Code:
 
E-AB-60760
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of Jurkat cells using Caspase-9 Polyclonal Antibody at dilution of 1:1000.
2 / 3
Immunohistochemistry of paraffin-embedded Human lung cancer using Caspase-9 Polyclonal Antibody at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Jurkat cells using Caspase-9 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human lung cancer using Caspase-9 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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E-AB-60760-60uL60uL£210.00
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E-AB-60760-120uL120uL£291.00
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E-AB-60760-200uL200uL£431.00
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  • Further Information
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Further Information

Abbreviation:
Caspase-9
Background:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
17kDa/30kDa/36kDa/46kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:100
Immunogen:
Recombinant protein of human Caspase-9
ObservedMW:
36kDa/46kDa
Purification method:
Affinity purification
Target Synonym:
CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
UNIProt ID:
P55211

Documents

References

  • https://www.spandidos-publications.com/10.3892/etm.2018.5718
  • https://link.springer.com/article/10.1007/s40200-022-01132-x