Elabscience

PPOX Polyclonal Antibody

Product Code:
 
E-AB-61932
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines using PPOX Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using PPOX Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using PPOX Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using PPOX Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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E-AB-61932-60uL60uL£210.00
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E-AB-61932-120uL120uL£291.00
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E-AB-61932-200uL200uL£431.00
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Further Information

Abbreviation:
PPOX
Background:
This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
50kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human PPOX
ObservedMW:
51kDa
Purification method:
Affinity purification
Target Synonym:
PPOX;PPO;V290M;VP
UNIProt ID:
P50336

Documents