Elabscience

L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

Product Code:
 
E-BC-K044-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
1 / 2
2 / 2
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E-BC-K044-M-48T48T£224.00
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E-BC-K044-M-96T96T£277.00
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E-BC-K044-M-500Assays500Assays£679.00
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  • Further Information
  • Documents
  • References
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Further Information

Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.12-7.0 mmol/L
Detection Type:
Colorimetric method
Inter cv:
3.5
Intra cv:
1.4
Result Type:
Quantitative
Sensitivity:
0.10 mmol/L
Test Principle:
Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.
Tested Sample Types:
Serum,plasma,cell culture supernatant,tissue,cells

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0092867422015379
  • https://www.nature.com/articles/s41423-022-00894-x
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8419046/
  • https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02521-y
  • https://www.sciencedirect.com/science/article/pii/S0223523423000818
  • https://www.sciencedirect.com/science/article/pii/S2352304222002331
  • https://link.springer.com/article/10.1186/s12964-022-00882-8
  • https://link.springer.com/article/10.1186/s12935-022-02845-y
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/
  • https://www.mdpi.com/1400748
  • https://www.sciencedirect.com/science/article/pii/S258900422101138X
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408126/