Elabscience

Recombinant E.coli Tryptophan Synthase β Chain/Trp B Protein (His Tag)

Product Code:
 
PKSQ050055
Product Group:
 
Recombinant Proteins
Supplier:
 
Elabscience
Host Type:
 
E.coli
Regulatory Status:
 
RUO
Shipping:
 
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
 
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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CodeSizePrice
PKSQ050055-10ug10ug£297.00
Quantity:
PKSQ050055-50ug50ug£498.00
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Further Information

Abbreviation:
Tryptophan Synthase β Chain;Trp B
Accession:
P0A879
Background:
Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.
Calculated MW:
43.8 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 um filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-His
ObservedMW:
40-50 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Thr2-Ile397
Target Synonym:
Tryptophan synthase beta chain;trpB
UNIProt ID:
P0A879

Documents