Elabscience

HA-Tag Monoclonal Antibody

Product Code:
 
E-AB-20008
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Mouse
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Clone:4G8
Regulatory Status:
 
RUO
Applications:
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western Blot analysis of 0.5ug HA fusion protein using HA-Tag Monoclonal Antibody at dilution of 1:10000.
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Immunofluorescence analysis of 293 cells transfected with a HA tag protein tissue using HA-Tag Monoclonal Antibody at dilution of 1:2000.
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Western Blot analysis of 0.5ug HA fusion protein using HA-Tag Monoclonal Antibody at dilution of 1:10000.
Immunofluorescence analysis of 293 cells transfected with a HA tag protein tissue using HA-Tag Monoclonal Antibody at dilution of 1:2000.

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E-AB-20008-30uL30uL£125.00
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E-AB-20008-60uL60uL£146.00
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E-AB-20008-120uL120uL£196.00
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E-AB-20008-200uL200uL£216.00
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  • Further Information
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Further Information

Abbreviation:
HA-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
Immunogen:
Synthetic Peptide
Purification method:
Protein A purification
Target Synonym:
HA epitope tag;HA1;HA2;hemagglutinin;Hemagglutinin HA1 chain;Hemagglutinin HA2 chain

Documents

References

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840182/
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.14207