Elabscience

H+K+-ATPase Activity Assay Kit

Product Code:
 
E-BC-K122-S
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
Reagent4:-20°C;Others:2-8°C
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CodeSizePrice
E-BC-K122-S-100Assays100Assays£197.00
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E-BC-K122-S-200Assays200Assays£277.00
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  • Further Information
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Further Information

Assay Time:
60 min
Detection Instrument:
Spectrophotometer
Detection Type:
Colorimetric method
Inter cv:
9.8
Intra cv:
4.4
Result Type:
Enzyme Activity
Test Principle:
ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.
Tested Sample Types:
Animal tissue,cells

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0378517321011091
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709249/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429807/
  • https://europepmc.org/articles/pmc9424734/bin/table5.docx?javascript_support=no
  • https://www.mdpi.com/1420-3049/27/16/5065
  • https://www.mdpi.com/1420-3049/27/18/5919
  • https://bmcpharmacoltoxicol.biomedcentral.com/articles/10.1186/s40360-022-00596-0