Elabscience

Flag-Tag Monoclonal Antibody

Product Code:
 
E-AB-48025
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3H9
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Monoclonal Antibody at 1:2000 dilution.
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Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Monoclonal Antibody at 1:2000 dilution.

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E-AB-48025-20uL20uL£110.00
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E-AB-48025-50uL50uL£143.00
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E-AB-48025-100uL100uL£177.00
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E-AB-48025-200uL200uL£210.00
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This product comes from: China.
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  • Further Information
  • Documents
  • References
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Further Information

Abbreviation:
Flag-Tag
Background:
FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Calculated MW:
45kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB: 1:5000-10000; IF: 1:500-2000,IP: 3ug/sample,FCM:0.2 ug
Immunogen:
Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
ObservedMW:
45kDa
Purification method:
Protein A/G Purified
Target Synonym:
DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag

Documents

References

  • https://pubmed.ncbi.nlm.nih.gov/36871040/
  • https://pubmed.ncbi.nlm.nih.gov/35194032/
  • https://pubmed.ncbi.nlm.nih.gov/35603194/
  • https://pubmed.ncbi.nlm.nih.gov/37010434/
  • https://pubmed.ncbi.nlm.nih.gov/32595669/https://pubmed.ncbi.nlm.nih.gov/36122636/
  • https://pubmed.ncbi.nlm.nih.gov/32188757/
  • https://pubmed.ncbi.nlm.nih.gov/32470978/
  • https://pubmed.ncbi.nlm.nih.gov/35746667/
  • https://pubmed.ncbi.nlm.nih.gov/33310083/
  • https://pubmed.ncbi.nlm.nih.gov/32745481/