Anti-monomeric Kusabira-Orange 2 pAb

WB - 1:1000 IP - 2 uL/Sample ICC - 1:500 IHC - 1:500 (Heat treatment is required)

CoralHue® Kusabira-Orange (KO) has been cloned from the stony coral, whose Japanese name is ?Kusabira-ishi?. Wild-type CoralHue® KO forms a brightly fluorescent dimer. CoralHue® KO has been carefully engineered to form a monomer, CoralHue® monomeric Kusabira Orange 1 (mKO1) that maintains the brilliance and pH stability of the parent protein. CoralHue® mKO2 is the mutant of mKO1 and has a feature of the rapid maturation. It absorbs light maximally at 551 nm and emits orange light at 565 nm. CoralHue® mKO2 can be used to label proteins or subcellular structures, or for reporter assay. PM051 is available for immunostaining of ?Fucci-G1 Orange? (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-G1 Orange encodes CoralHue® monomeric Kusabira-Orange2 (mKO2) fused to a part of human Cdt1 (hCdt1: Cdc10 dependent transcript 1). It is possible to use PM051 for Fucci transgenic strain, B6.Cg-Tg(Fucci)596Bsi mice which express Fucci-G1 Orange.

100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Recombinant mKO2

This antibody reacts with monomeric Kusabira-Orange 2 on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.

1 year

This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant monomeric Kusabira-Orange 2.

Kusabira-Orange 2