MBL

Anti-Oct3/4 (Mouse) pAb

Product Code:
 
MBL-PM048
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Rabbit
Antibody Isotype:
 
Affinity Purified Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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CodeSizePrice
MBL-PM048100 uL£433.00
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Further Information

Applications:
WB - 1:1000 (chemiluminescence detection system) IP - 2 uL/300 uL of cell extract from 3 x 106 cells ICC - 1:500 IHC - 1:500
Background:
The POU family transcription factor Oct-3/4, termed as Oct-3 or Oct-4, encoded by Pou5f1, is expressed in totipotent/pluripotent early embryonic cells. It is also expressed in embryonic stem (ES) cells and embryonal carcinoma (EC) cells, but its expression diminishes when these cells differentiate and lose pluripotency. Oct3/4 contains three functionally characterized domains, the transcriptional activation domain of N- and C-terminal region and the POU DNA-binding domain. The POU domain binds to an octamer sequence, ATTTGCAT. Several target genes of Oct3/4, such as Sox2, contains an octamer element capable of binding Oct3/4. These sites are important for transcriptional activity. Induced pluripotent stem (iPS) cells can be generated from mouse embryonic or adult fibroblasts by induction of four factors, Oct3/4,Sox2, c-Myc, and Klf4. Oct3/4 regulates a expression of Tcl1 (T cell lymphoma break point) and Nanog and contributes to cell proliferation and stabilization of cell pluripotency. Two transcription factors Oct3/4 and Sox2 works together to control a transcriptional regulatory network that regulates the expression of other essential genes.
Formulation:
100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 5460 Mouse: 18999
Immunogen Translated:
Recombinant mouse terminal Oct3/4N (1-134 aa)
Reactivity:
This antibody reacts with Oct3/4 on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with recombinant N-terminal of mouse Oct3/4 corresponding to 1-134 aa.
Target:
Oct3/4

Documents

References

1) Boer, B., et al., Nucleic Acids Research 35, 1773-1786 (2007) 2) Takahashi, K., and Yamanaka, S., Cell 126, 663-676 (2006) 3) Chew, J., et al., Mol. Cell Biol. 25, 6031-6046 (2005) 4) Niwa, H., et al., Cell 123, 917-929 (2005) 5) Saijoh, Y., et al., Genes to Cells 1, 239-252 (1996) 6) Shimazaki, T., et al., EMBO. J. 12, 4489-4498 (1993) 7) Okamoto, K., et al., Cell 60, 461-472 (1990)