MBL

Anti-His-tag pAb-Agarose

Product Code:
 
MBL-PM032-8
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Immunoprecipitation (IP)
Shipping:
 
4°C
Storage:
 
4°C
1 / 2
2 / 2
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CodeSizePrice
MBL-PM032-8Gel: 200 uL£307.00
Quantity:
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  • Further Information
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Further Information

Applications:
IP - 20 uL
Background:
Expression vectors containing a protein and a tag peptide are commonly used. His-Tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-Tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
Conjugate:
Agarose
Formulation:
320 ug of anti-His-tag polyclonal antibody covalently coupled to 200 ul of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.09% sodium azide) for a total volume of 400 ul.
Immunogen Translated:
6xHis synthetic peptide carrier protein binding
Reactivity:
This antibody recognizes His-tagged protein on Immunoprecipitation
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated?6xHis synthetic peptide.
Target:
His

Documents

References

1) Kajikawa, M., et al., J. Virol. 86, 5288-5296 (2012) 2) Shibata, Y., et al., J. Biochem. 150, 679-686 (2011) 3) Takemoto, M., et al., Cereb. Cortex 21, 1925-1934 (2011) 4) Kojima, K., et al., Genes Cells 14, 1155-1165 (2009) This antibody is used in these references.